ABSTRACT
Objective To study the chemical constituents from the Stichopus variegatus .Method The compounds were isolated and purified by silica gel column chromatography ,Sephadex LH-20 gel filtration and recrystallization .The structures of these compounds were identified through chemical reactions and spectral analyses ,such as IR ,1 H ,13C NMR ,MS .Results Three compounds were identified as 3-O-[3-O-methyl-β-D-glucopyranosyl (1→3)-β-D-xylopyranosyl (1→3)-β-D-glucopyranosyl (1→2)-β-D-xylopyranosyl]-holosta-7 ,24-diene-3β,23S-diol ,named as variegatuside C ;3-O-{3-O-methyl-β-D-glucopy-ranosyl(1→3)-β-D-xylopyranosyl(1 → 4)-β-D-glucopyranosyl (1 → 2)[β-D-glucopyranosyl (1 → 4)]-β-D-xylopyranosyl-holosta-8-ene-3β, 23S-diol ,named as variegatuside D ;3-O-{3-O-methyl-β-D-glucopyranosyl(1→3)-β-D-xylopyranosyl(1→4)-β-D-glucopyranosyl (1→ 2 ) [3-O-methyl-β-D-glucopyranosyl (1 → 3 )-β-D-glucopyranosyl (1 → 4 )]-β-D-xylopyranosyl}-holosta-9-ene-3β,23S-diol , named as variegatuside E .Conclusion All three compounds are new compounds .
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Bioassay-guided isolation using an in vitro assay testing for anti- schistosomiasis yielded a novel triterpene saponin, asparagalin A, from the n-butanol extract of the roots of Asparagus stipularis Forssk., Asparagaceae. The structure was elucidated by spectroscopic analysis and chemical transformations. Administration of asparagalin A resulted in a retardation of worm growth and locomotion at the first day and showed a significant activity of egg-laying suppression at 200 µg/mL concentration.
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In an effort to identify the chemical constituents of fruiting bodies of Fomitopsis pinicola, a new lanostane triterpene glycoside, designated as fomitoside K, has been isolated from its methanolic extract. Its chemical structure was assigned on the basis of various spectroscopic studies.
Subject(s)
Coriolaceae , Fruit , MethanolABSTRACT
Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity.Methods Triterpene glycosides from H.scabra were separated and purified by chromatography on DA-101,silica gel,and reversed-phase silica gel column,as well as RP-HPLC.Their structures were elucidated on the basis of spectral data and chemical evidence.Results Three triterpene glycosides were identified as scabraside D (1),fuscocineroside C (2),and 24-dehydroechinoside A (3).Their inhibition on P-388,A549,MKN-28,HCT116,and MCF-7 cells were significant.Conclusion Scabraside D (1) is a new triterpene glycoside,and compounds 2 and 3 are isolated from H.scabra for the first time.The glycosides 1-3 show the in vitro cytotoxicity against five human tumor cell lines in comparison to 10-hydroxycamptothecin.
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Objective: To study the constituents from the sea cucumber, Stichopus variegatus. Methods: The crude saponins of S. variegatus were desulfated with pyridine-dioxane (1:1), the desulfated products were separated by multi-chromatography to afford two compounds. Results: On the basis of chemical methods and spectral evidences, especially 2D-NMR and ESI-MS, these two compounds were identified as 3-0-[β-D-xylopyranosyl-(1→2)-4′-O- sulfate-β-D-xylopyranosyl]-holosta-9-ene-3β,12α,17α, 25β-tetraol sodium salt, named as variegatuside A (1) and 16β-acetoxy-3-O-β-D-xylopyranosyl-holosta-22,25-epoxy-9-ene-3β, 12α,17α-triol, named as variegatuside B (2). Conclusion: Compounds 1 and 2 are new compounds.
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Objective: To study the constituents from the sea cucumber Holothuria nobilis. Methods: The compounds of the sea cucumber were separated by multi-chromatography and their structures were elucidated by physico-chemical and spectral analyses. Results: Two new triterpene glycosides were isolated and their structures have been deduced by extensive spectral analysis (IR, 2D-NMR, and ESI-MS) and chemical structures as 3-O-{3″″-O-methyl-β-D- glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D- quinovopyranosyl-(1→2)-4′-O-sulfate-β-D-xylopyranosyl} -holosta-22, 25-epoxy-9-ene-3β, 12α, 17α, 25β-tetraol- sodium salt (nobiliside I, 1) and 3-O-{β-D-quinovopyranosyl-(1→2) 4′-O-sulfate-β-D-xylopyranosyl}-holoshillaside 18 (16)-lactone-22, 25-epoxy-9-ene-3β, 12α, 17α-triol sodium (nobiliside II, 2). Conclusion: Two aglycons are both new compounds named nobiliside I and nobiliside II.
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Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .
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Objective To investigate the chemical constituents of the sea cucumber Holothuria impatiens collected from the South China Sea.Methods The pure compounds were isolated by extraction,partition and multi-chromatography.Their structures were elucidated on the basis of spectral analysis and chemical evidence.Results Three triterpene glycosides were isolated and identified as holothurin A1(Ⅰ),holothurin A(Ⅱ) and pervicoside C(Ⅲ),together with a physiological substance of adenosine(Ⅳ).Conclusion All were isolated from Holothuria impatiens for the first time.Three glycosides showed significant cytotoxic activities.